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Planning a Perfect Desert (Tortoise) Getaway

A desert tortoise is in safe hands during translocation.

A desert tortoise is in safe hands during translocation.

My boots are sitting in the hallway covered in dust. Not from lack of use, I might add. But following such an inspiring field experience in the Mojave Desert, where I released tortoises to the wild, it just seemed too soon to wipe away the dusty memories of my desert adventure.

If you work at a desk most of the time, like I do, you’ll understand that getting out into the field can be a rare and fleeting opportunity. Fortunately for me, my job takes me on walkabout to visit our field programs about once every couple of months. Each trip has a dedicated mission, from delivering vital field equipment to planning field operations alongside remotely based staff, and each trip has the added bonus of bringing me joy in connecting with the animals, people, and places where we work.

On this last trip, my trifold mission was to deliver a brand-new four-wheel drive truck to our Desert Tortoise Conservation Center, assist in a long-planned translocation of desert tortoises to the Greater Trout Canyon area just west of Las Vegas, and take staffer Julie Marshall on a memorable professional training experience. Julie works diligently overseeing our Institutional Animal Care and Use Committee, ensuring all our projects follow the highest animal welfare standards, and this trip would give her the golden opportunity to see our tortoise conservation efforts in action.

Julie Marshall radio tracks a desert tortoise.

Julie Marshall radio tracks a desert tortoise.

Our first day was spent with the staff at the Center, carefully preparing animals for their move to the wild. Each tortoise has undergone a meticulous health screening and testing over the past year to ensure its overall condition will not compromise its chances for survival. Carrying out the finishing touches, we attached radio transmitters to each of the 32 tortoises destined for freedom. The translocation effort serves two main purposes: population augmentation and research. The population of wild tortoises in the Greater Trout Canyon area has been in decline for several years. The translocated animals are expected to bolster the population and, at the very least, slow the decline in numbers. Research includes checking on the released tortoises at regular intervals to determine how they are adapting to their new environment.

Waking uncomfortably early at 4 a.m. the next day, we headed out to meet the staff, volunteers, and members of other agencies (NDOW, FWS, USGS), assisting in the translocation. After a briefing and some quick training in the use of handheld GPS units, we drove off in convoy with our precious cargo. As dawn was breaking, each tortoise received a subcutaneous injection of liquid to ensure proper hydration, and each person, carrying a tortoise in a tote, navigated to a target GPS location to make a “drop”. Fanning out across the desert landscape, our work was accomplished swiftly in the cool morning air, some tortoises remaining still until we left their sides, others trotting off into the near distance. We all left wondering, what next?

Our third and final day started just as uncomfortably early as day two and focused on finding out what next. While I caught up on center operations, Julie accompanied our field technicians to learn how to radio track tortoises. Hiking across the desert terrain following the beeping sound of an animal’s transmitter is tough work, but the payoff when you find an animal is exhilarating. After animals are translocated, they often make longer-range movements than normal in exploring their new environment, so it is key for us to follow them closely during their first few weeks of release so we don’t lose anybody.

I’m happy to report all tortoises were relocated, and I’m grateful that my job involves working with the dedicated members of the Desert Tortoise Conservation Center team who are making conservation happen and who are all infinitely better morning people than I!

Allyson Walsh is an associate director in the Applied Animal Ecology Division of the San Diego Zoo Institute for Conservation Research.

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“Fiddler on the Roof” Meets Conservation Biology

San Clemente Loggerhead Shrike

How does the busiest, most critical part of the year—the breeding season—even begin for the shrikes and staff of the San Clemente Loggerhead Shrike Breeding Program?

Since 1989, the San Diego Zoo Institute for Conservation Research, in conjunction with the United States Navy, has been making great efforts to recover endangered San Clemente loggerhead shrikes by breeding them in aviaries and releasing juveniles into the wild. Because of the efforts of the Zoo, the Navy, and those of partner conservation organizations, this shrike subspecies, which occurs only on Navy-owned San Clemente Island off the coast of California, has increased from a population of 14 individual birds in 1998 to 65 breeding pairs in 2013. Although there is much work yet to be done, the recovery program’s success story is well known. But what exactly goes into such a project? More interestingly, what goes into the busiest, most critical part of the year for the program? How does the busiest, most critical part of the year—the breeding season—even begin for the shrikes and staff of the San Clemente Loggerhead Shrike Breeding Program?

The breeding effort begins in January, when we receive a very exciting document delivered by Tandora Grant, the San Clemente loggerhead shrike’s studbook keeper. It is her responsibility to use genetic and demographic statistics to determine which of our shrikes to breed each year. Two important factors that come into play during Tandora’s matchmaking are the representation of our birds’ genes in the wild population’s gene pool and each of our bird’s personal breeding history. It is vital to balance these two factors to maximize the positive impact our program has on the recovery of the shrike; too few juveniles to release at the end of the season results in a low probability of their surviving to breed the next year, but releasing many genetically invaluable juveniles is potentially detrimental to the recovery of the species. Think Fiddler on the Roof meets conservation biology; the document Tandora delivers is the year’s breeding recommendations, and it contains the season’s breeding pairs, whether the birds are happy with her choices or not! More often than not, the birds are happy, but the document also contains alternate pairings should any of the chosen shrikes display a lack of motivation when it comes time to court each other.

In the first week of February, after we have prepared the breeding aviaries for the upcoming season, the select females are moved into aviaries adjacent to their males in a logistics puzzle that has been appropriately named “The Big Move.” You can imagine how hard it is to place 12 to 15 specific pairs next to each other in appropriately outfitted breeding enclosures when we have a flock size of over 60 birds and a grand total of about 80 enclosures! Though it is sometimes difficult and requires lots of planning, a little bit of luck, and plenty of cooperation from the shrikes, The Big Move is important, because it is designed to imitate the natural movement of wild shrikes.

In the wild, male and female San Clemente loggerhead shrikes maintain exclusive and solitary winter territories; however, come breeding season, females leave their winter grounds to search for attractive mates. By moving a specific year’s breeding males into their breeding enclosures and the female’s into enclosures adjacent to their chosen mates, we aim to simulate the female’s discovery of her mate. Once the breeding pairs have been placed in their adjacent enclosures, they have entered the “pre-pair” phase of the breeding season. This is the time for the males to court the females by displaying, singing, nest building, and most importantly, feeding them lots of bugs! If all goes well, we will be able to move on to the next phase—pairing.

Henry Fandel is a research associate for the San Diego Zoo Institute for Conservation Research.

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Perfect Parrotbill Puppets

Click on the link to watch this parrotbill hatch.

Click on the link in the first paragraph to watch this kiwikiu (parrotbill) hatch.

The Maui Bird Conservation Center is pleased to announce the hatch of our second kiwikiu (Maui parrotbill) chick of the breeding season. The chick hatched on April 11 at 11 a.m., and I was lucky enough to see the chick hatching and took this short video clip: Kiwikiu (parrotbill) hatching.mov

The kiwikiu is an endangered, endemic Hawaiian honeycreeper only found in a small range on the eastern slopes of the Haleakala volcano on Maui. This species has been notoriously difficult to breed in captivity, but the Hawaii Endangered Bird Conservation Program has been having more luck in producing chicks in the last few years (see Raising Maui Parrotbills).

A puppet "parent" feeds the new kiwikiu.

A puppet “parent” feeds the new kiwikiu.

The kiwikiu is a very intelligent species, and we take many steps to keep the birds from imprinting onto humans. We use a hand puppet during feeds as soon as the young chick’s eyes start to open, and this year we thought it was time we “upgraded” our hand puppet.

At the Maui Bird Conservation Center’s Open House last November, I met a lovely local lady, Alyson Danford, who obviously has a real passion for the native wildlife of Hawaii. Alyson grew up on the Big Island of Hawaii but has now lived on Maui for more than 28 years. Alyson created a beautiful quilt of the kiwikiu among the native Acacia koa tree, and she donated this wonderful gift to our program during the open house. I immediately thought of Alyson about making the new hand puppet and contacted her about the project.

Alsyon stands in front of a quilt she made, inspired by the alala's hoped-for return to the wild.

Alyson stands in front of a quilt she made, inspired by the alala’s hoped-for return to the wild.

Alyson was very excited to help us even though she had never made anything like that before, and after a visit to our facility, she came up with two new hand puppets for our program! It was perfect timing when Alyson had the new puppets ready for our newly hatched chick.

We are extremely grateful to Alyson for donating her time and creativity to help us toward our mission of protecting the native birds of Hawaii. Alyson, Mahalo nui loa. Me ka aloha pumehana.

Amy Kilshaw is a research associate at the San Diego Zoo’s Maui Bird Conservation Center. Read her previous post, Nene Come Home.

6

Catering to Animals in the “Back 40”

DuikersDuring my years at the San Diego Zoo, Iʼve seen thousands of guests watch keepers with as much curiosity as they give the animals. Eyes follow that besmudged, khaki-clad person, arms loaded down with buckets or pulling a wheelbarrow full of unidentifiable and smelly stuff. Where do keepers disappear to in between those brief periods of travel? What is behind that big gate or inside that noisy building? Would it surprise you to know that there are very large areas of the Zoo that are what we call off exhibit?

These areas usually house animals that canʼt be on exhibit for some reason or other: maybe they are in need of special vet care, theyʼre getting an update to their exhibit (or maybe a brand-new one!), they are part of one of our conservation breeding programs, or they just need a day off to relax in a hammock and snack on some treats. The area of the Zoo I work in caters to all of those needs and more! This area is only briefly visible from the top level of one of our popular double-decked bus tours as they pass the Elephant Care Center in our Elephant Odyssey. We affectionately call this place the Back 40.

One place in our Back 40 where I spend a good amount of time is called the Shipping Pens. Picture a place where the walls can move and the floor can drop out and where we can take care of the special needs of just about any ungulate species (hoofed animal) that we have at the zoo! One of the biggest functions of the keeper at Shipping Pens is the shipment of animals, both incoming and outgoing. When the Zoo’s curator sends us a move notice for an animal, we usually move it to Shipping Pens, where the keeper can work with our veterinarian staff to make sure the animal gets all of its necessary pre-shipment exams, gets used to being in a crate or trailer, and anything else we can do to make sure the animal will have a stree-free move. If that animal(s) is/are internationally bound, there is usually a quarantine period that is monitored by a US Department of Agriculture veterinarian, whom we also work with.

Soemmerring's gazelles keep an eye on their keeper.

Soemmerring’s gazelles keep an eye on their keeper.

The Shipping Pens keeper also takes care of special vet cases. Iʼve helped reattach a horn on a male Soemmerringʼs gazelle when he broke it off sparing with another male in his exhibit. I have found ways to get a male yellow-backed duiker to take his medication when he didnʼt want to eat anything. I have manually restrained a male pronghorn so the vet could clean out a leg wound and administer topical medication. Iʼve helped bottle feed a baby royal antelope when her mother was unable to nurse her well enough. At the Shipping Pens, I have helped nurse animals back to health and have provided them with a nice, quiet place in which to do so.

This area also has the unique distinction of being one of the most action-packed areas in the Zoo. In most areas of the Zoo, keepers might perform up to 10 animal moves a month, if itʼs busy, but in our area, there was one month when we moved over 40 animals! Keepers who work this area need to know how to work with any species of hoofed animal that visits.

If you think whatʼs happening on exhibit is cool, itʼs nothing compared to what happens behind that big gate! Sure, thereʼs an awful lot of poop to clean up, but there is also tons of really interesting stuff getting done at any given time, in any off-exhibit area. And since you canʼt come visit us, I thought Iʼd bring a little bit of our world to you! So next time you find yourself on the top level of the tour bus going through Elephant Odyssey, take a second to look left, over the big gate, and wave at us there in the Back 40!

Ashley Roberts is a keeper at the San Diego Zoo.

46

Polar Bears: Back to Normal

Tatqiq sniffs the air after a good roll in the mulch this morning.

Tatqiq sniffs the air after a good roll in the mulch this morning.

It’s been a whirlwind few months for all of us at the San Diego Zoo’s Polar Bear Plunge! It began on January 2 and is only just now settling into what we all refer to as normal! On January 2, we re-introduced Chinook to Kalluk and Tatqiq. It became very apparent in the previous week that Chinook was very interested in being with Kalluk. The three fabulous bears took up together as though they had not been apart for the months we were waiting to see if Chinook would give birth. All of us who work with our bears thought now we just wait for breeding season to start. The wait was NOT long!

On January 4, we came in to find it had begun and Kalluk and Chinook were inseparable. After a week of togetherness, it was all over for Chinook, but that was when Kalluk’s breeding drive took off. During the months that followed, we spent lots of time preparing foods that our boy would find appetizing to help keep any weight on him while nature takes over and he loses his appetite and seems to endlessly search for other mates. We also try different management techniques to see if any help to ease the road for Kalluk.

In the wild, male polar bears also go off their food in an effort to find receptive females. They, too, can lose an enormous amount of weight during this time, but adult males can make it up after breeding as they hunt yearlong on the ice and don’t have the need to fatten up to survive months in the den producing milk for cubs! However, with the summer ice beginning to disappear earlier, and knowing that males can be in breeding mode until June, it is worrisome to know what effects this could have on our wild male populations.

Polar bear breeding season can last into June, so although it is still possible that Chinook and Kalluk could breed again, Chinook’s behavior indicates that it is not likely. Kalluk is also showing fewer behaviors to indicate this as well. What does all this mean? We don’t have the exact answers, but it is likely that when the breeding occurred in January, Chinook ovulated, and if the egg was fertilized, she would not have the biological need to breed again. If this were in the wild, she would have begun hunting and storing as much body fat as possible to rear her cubs. It is interesting that so far this year she has been gaining weight more so than any year previously at this time.

The actual weight of polar bear cubs would not have a significant impact on their mother’s weight. Cubs are, after all, less than 2 pounds (1 kilogram) at birth. But we are optimistic that Chinook’s weight gain is an indicator that her body is holding on to every calorie she would need in the future for cub rearing.

Kalluk and Tatqiq have renewed their bond and can now be seen wrestling and playing together. Chinook is spending her days relaxing, eating carrots, and taking those beautiful, long soaks in the pool. She has quite a “full” figure these days; actually, she is gorgeous! So the warm San Diego summer will have her lounging and soaking most of the time. She will, of course, be given the option of staying in the air-conditioned bedrooms so you may not see her as much as in previous summers.

We know the question is already there: is she or isn’t she? We don’t know.  We will continue to work on research to give us better answers, continue to monitor Chinook’s behavior to provide for our girl exactly what she needs, and keep all fingers and toes crossed. We’re all getting pretty good at that!

JoAnne Simerson is a senior keeper at the San Diego Zoo. Read her previous post, Polar Bears: Hormones.

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Panda Cub: Rolling and Tumbling

The little cutie seems to imitate his father's relaxed eating style.

The little cutie seems to imitate his father’s relaxed eating style.

As the San Diego Zoo’s panda cub, Xiao Liwu, gets more and more confident in his enclosure, we are beginning to see some fun new behaviors from him and his mother, Bai Yun. So far, at least once a day the cub is coming down out of the tree to get some exercise with his mom and possibly nurse. The time frame and duration of his stay out of the tree has varied from day to day. Xiao Liwu enjoys coming down and jumping on his mom’s back and wrestling with her, and Bai Yun has been super patient and puts up with quite a bit of biting from her little one.

Bai Yun has been extremely relaxed these days and is maintaining a stable weight of 230 pounds (104 kilograms). She is not too rough with her cub and is showing off those mommy moves that we all love so much. As Xiao Liwu is teething and trying out the bamboo, Bai Yun has been surprisingly calm about him getting into her food and trying new pieces. When he initiates a wrestling match, she has been very obliging.

Two days ago, the cub gave our guests a heart-stopping moment—he fell out of the tree from about 20 feet (6 meters). As keeper Jen and I were talking, the cub was in the tree playing on a new branch and trying out some new moves. We looked up for a second, and Xiao Liwu rolled out of the tree! Wu never made a sound—just got right back up and continued playing. He’s moving just fine, and Bai Yun was not alarmed at all by the little oops he made from the tree.

Now I know some of you will be wondering if we need to check him or why we didn’t grab him, and the answer is simple: he’s tough! We did not see any limping or stress behavior from either Mom or cub. Panda cubs are designed to make those climbing mistakes at this young, bouncy age. That layer of baby fat helps, too!

So keep on watching and come see us soon. Just a word to the wise: there is NO schedule for when the cub comes down to play, so please remember to give him some time.

Anastasia Horning is a panda narrator at the San Diego Zoo.

UPDATE: The main panda viewing area is currently closed as we make modifications to it. Bai Yun and Xiao Liwu have been moved back to the north exhibit, where they can be viewed by guests. Pandas Gao Gao and Yun Zi are off exhibit during this time.

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Mr. Wu on View

T13_0244_019It has been about a month since giant pandas Bai Yun and Xiao Liwu have moved to the main viewing exhibit, and what a fun time it has been for San Diego Zoo guests and for our little panda boy! Mr. Wu has adjusted to the new exhibit very well, spending his days exploring every inch of his new habitat, from the ground to the trees. And when he explores the trees, he goes way up high!

Panda cubs are great climbers, and in the wild, high in the trees is the best place for cubs to stay safe. Mr. Wu can be seen lounging 20 to 30 feet (6 to 9 meters) up in the pine tree throughout the day. He is a strong climber and gets up and down with ease. Keepers have also recently installed grass sod in the exhibit, and Mr. Wu is having a great time ripping up the sod and playing with sod chunks.

Xiao Liwu continues to grow like a weed and weighs about 30 pounds (13.6 kilograms). He turned nine months old today! Although he is not yet eating a lot of solid food, he does like to chew on bamboo and really enjoys applesauce. Mr. Wu is still a mellow guy with a sweet personality, and we are all enjoying seeing him grow up and become a “big bear.”

Elizabeth Simmons is a keeper at the San Diego Zoo. Read her previous post, Panda Cub Learning Routine.

3

Condor Chick Watching: Age 30 to 45 Days

Cuyamaca does some preening in the nest box.

Cuyamaca does some preening in the nest box.

At about one month of age, our California condor chick Cuyamaca (pronounced “Kwee-ah-MACK-ah” and meaning “through the clouds” in Kumeyaay), should weigh around 4.4 pounds (2 kilograms). The parents, Sisquoc and Shatash, may start leaving the chick alone overnight, sleeping near the nest. If the weather is still cool or it’s raining, they may continue to brood overnight until the weather improves. Even though the parents are increasing their time away from the chick, they remain VERY vigilant and protective of their nest and ESPECIALLY their chick. Some field biologists have even seen wild condor parents chasing black bears away from the nest area!

Up until now, the chick has been scooting around the nest on its tarsal joints. We refer to that as a tarsal crawl. It’s not uncommon, at this age, to see the chick standing all the way up on its feet, teetering around the nest, holding its wings out for balance. As its legs get sturdier, the chick may even approach the parent, begging for food. The wing-begging behavior we’ve been seeing will get more pronounced: lots of wing-flapping, head-bobbing, and trying to position itself in front of the parent.

It is possible that the parents, who are offering larger quantities of food per feeding session, might be providing a small amount of fur/hair in the chick’s diet. (Part of the adults’ diet includes mammals, like rats and rabbits.) Condors can digest just about every part of the animals they eat, except for fur. This fur accumulates in the digestive tract and is eventually regurgitated as waste. We refer to this as casting. A condor’s cast is composed of predominantly fur, whereas a cast from an owl has fur and bones; owls can’t digest bones, but condors can. We have seen condor chicks cast hair pellets as young as three weeks of age. When the chick casts, it throws its head forward several times, mouth open, until the pellet is ejected from its mouth. It can look like the chick is in trouble, but it is perfectly normal and good for the chick.

At around 45 days of age, Cuyamaca will get its first health exam. We will obtain a blood sample for the lab to make sure the chick is healthy and send a portion of this sample to a lab in the Genetics Division of the San Diego Zoo Institute for Conservation Research. From this blood sample, the geneticists can determine if Cuyamaca is male or female. Also during the exam, we will weigh Cuyamaca (the chick should weigh between 7.7 and 8.8 pounds or 3.5 and 4 kilograms), and we will inject a transponder chip as a form of identification. It’s the same kind of chip you can get for your dog or cat at the veterinarian. Most importantly, this exam allows us to administer a vaccine for West Nile virus, a disease that originated in Africa and was accidentally introduced to North America by humans. North American animals, including condors, usually don’t have a natural immune response to West Nile virus, so we are trying to give our chicks as much of a head start as we can.

This exam will be the first time that Cuyamaca will see humans, so it will naturally be disturbing for the chick. We try to be as quick as we can (9 to 10 minutes) to minimize the disturbance. Additionally, we will keep Cuyamaca covered with a towel to reduce its exposure to humans and to provide it a bit of security. Sisquoc and Shatash are usually away from the nest when we perform the procedure to keep them as calm as possible, as well. We have to keep in mind that we don’t want Cuyamaca to become accustomed to or feel reassured by our presence; we want it to be a wild condor, uninterested and wary of humans, so that it may someday fly free in California, Arizona, or Mexico.

Cuyamaca will look very large at this age compared to how big it was at hatch, but remember that it is still less than half of its adult weight. There is much more growth and fun to come on Condor Cam!

Ron Webb is a senior keeper at the San Diego Zoo Safari Park. Read his previous post, Condor Chick Watching: Age 3 to 4 Weeks.

4

A Trip Down Memory Lane

Rebecca tells students about the work she does helping endangered Hawaiian birds.

Rebecca tells students about the work she does helping endangered Hawaiian birds.

While it has been a long time since I stepped into a classroom, the second I walked onto Pahala Elementary School’s campus a flood of memories of my own school days came rushing back. I remember coming into the first class of the day and still wanting time to chat with my friends. I remember the small tables and chairs that I know I used to fit into, though now it’s difficult to imagine. And while I remember the class bells ringing in school, yesterday I was very nearly shocked out of my skin when the bell signaled the start of class. I guess that’s the sort of thing you never notice as a kid.

That morning I had the pleasure of assisting Robin Keith, a member of the Conservation Education Division at the San Diego Zoo Institute for Conservation Research, in administering an essay contest to the sixth- and seventh-grade classes of Pahala Elementary and Ka‘u High School in Pahala, Hawaii. This essay contest was designed to discover a student’s own interpretation of, and experiences with, wildlife. The information will help guide our conservation education and outreach programs in support of our Hawaii Endangered Bird Conservation Program. Some students struggled at the beginning, not really sure what to write about, but in the end they all submitted great stories. Two winners will be chosen at random, and that student will be taking his or her entire class on a field trip to the ‘Imiloa Astronomy Center of Hawaii.

Students work on their wildlife conservation essays.

Students work on their wildlife conservation essays.

After the essay portion of the class, Robin spoke about current conservation issues facing Hawaii and about techniques used at the Keauhou Bird Conservation Center (KBCC) for saving native Hawaiian birds. Then it was my turn to field any questions that the students had about KBCC. I have spent the past six years working with some of the most endangered and difficult-to-rear bird species in Hawaii, but when it came to commanding the attention of 12 and 13 year olds, I was a bit daunted. Usually, when I’m presenting information about my job, I’m in my workplace with every conceivable prop and medium available to showcase the native birds. Here, however, I was standing at the front of the classroom with nothing but my strong voice, great bird conservation information, a smile, and enthusiasm for my job! In the end, I hope the students walked away with a great writing exercise and some valuable information about Hawaiian bird conservation. I walked away from the campus hoping that I had planted at least one seed of love and respect for native Hawaiian wildlife.

I must send out a very big mahalo (thank you, in Hawaiian) to the teachers of Pahala Elementary and Ka‘u High School for allowing Robin and me to invade their classes and for their enthusiasm in teaching their students environmental education. Another very big mahalo to the wonderful students, who had excellent questions about the birds and embraced the essay-writing challenge. We look forward to future collaborations with students and teachers on the Big Island as we work to foster pride and support for conservation of Hawaii’s natural heritage.

Rebecca Espinoza is a research associate at the San Diego Zoo’s Keauhou Bird Conservation Center.

2

Tortoise in the Glass: Evaluating Health Problems

To you, a typical tortoise might look like this:

desert tortoise adult

But to me, a tortoise may also look like this:

desert tortoise tissue samples

I’m a veterinary pathologist, which means I spend a lot of quality time looking through a microscope at slides with tissues to try to evaluate health problems that show up as changes in those tissues. I can find dying cells, inflammation, various pathogens, scarring, thinning, thickening, bleeding, tumors, strange crystals, and unusual pigments. All of the changes help us understand the health problems affecting an animal.

At the San Diego Zoo Institute for Conservation Research, I work exclusively on tortoises that have died at the Desert Tortoise Conservation Center. Why bother? Well, it turns out that one of the best ways to figure out what health indicators most accurately indicate disease is to compare the information from the live tortoise to the changes we see in the tissues if the animal dies. The more we know about which tools work to predict severity and type of disease, the faster and more precise we are at identifying and helping animals at risk.

To get information from enough tortoises to allow good conclusions to be drawn, I need to look at a lot of slides. Since 2009, over 4,500 slides have been made of desert tortoise tissues, providing an invaluable resource for the understanding of disease in desert tortoises. Since November 2012, I’ve been describing the changes I see so that they can be correlated to what was found in the live animal. Thankfully, I haven’t been working all alone; Dr. Lily Cheng, another veterinary pathologist, volunteered to spend two whole months staring at a mountain of desert tortoise slides. Between the two of us, we’ve done more than 3,000 slides belonging to over 250 tortoises!

Are you curious about what sorts of things we see? Good! We are always on the lookout for bacteria or viruses that cause that most feared of tortoise infections: upper respiratory disease. This is more than just a head cold like people get and is a big factor in tortoise population decline. Some savvy souls may note that no light microscope can show an individual virus particle (you really need an electron microscope for that, since viruses are smaller than the wavelength of visible light). Conveniently, however, some viruses clump together to form rafts of virus particles. These are big enough to see with a microscope, just as you can see a patch of lawn even if you are too far away to pick out a single blade of grass. The virus most common and dangerous in tortoise respiratory disease (herpesvirus) forms these aggregations in the nuclei of cells, and they are called intranuclear inclusions.

Below are some cells from a tortoise that had severe upper respiratory disease. On the left side of the picture, you can see normal nuclei: round or oval purple shapes that look very speckled, like chocolate chip cookies. On the right side of the picture, the nuclei are bigger and have clumps of magenta in the center surrounded by a clear rim. They no longer resemble chocolate chip cookies at all. Those magenta blobs are viral inclusions from herpesvirus!

Herpes inclusions

The work continues at a good pace, and there are only about 1,300 slides left to look at. They weigh almost 7 kilograms (15 pounds) altogether. Wish me luck!

Kali Holder, D.V.M., is a postdoctoral associate in the Wildlife Disease Laboratories for San Diego Zoo Global.