Animal Stories

Animal Stories

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Breeding Snow Leopards: Lab View

Does Beau have what it takes?

Last month, Mammal Keeper Todd Speis blogged about the introduction and subsequent breeding of Anna, the Zoo’s snow leopard, and her new mate, Beau (see Snow Leopards: Love at Second Sight?). Our Reproductive Physiology Department staff first met Beau in 2006 at his previous home in the Santa Barbara Zoo. We were there to perform a semen collection procedure to assess his potential fertility, not knowing at the time that one day he would come to the San Diego Zoo. Beau was a gorgeous snow leopard with motile sperm.

Back in San Diego, Anna and her long-time mate, Everett, were a compatible breeding pair, but despite their efforts she never became pregnant. We were asked to examine the pair to try to diagnose the problem and offer a possible solution. The first, and least invasive step, was to analyze Anna’s estrous cycles. Keepers collected fecal samples, froze them, and sent them to our Endocrine Laboratory, where Alan Fetter charted her cycles by measuring her fecal estrogen and progesterone levels.

We could see that she was breeding at the appropriate time in her cycle, and she was ovulating normally. When her progesterone remained elevated after each of two breeding cycles, we were hopeful that she was pregnant. But each time her progesterone plummeted back to baseline within two months, indicating that she had experienced a pseudopregnancy (also known as a false pregnancy). This condition is not uncommon among mammals, especially in carnivores. In most cases, pseudopregnancies last half to two-thirds of the length of a normal pregnancy, which is what we observed with Anna. A pseudopregnancy occurs when a female ovulates but does not conceive. For a few weeks her body produces hormones to support a pregnancy even though there is no embryo present. Eventually, with no communication from an embryo, the female’s body returns to normal, and she cycles again.

After two documented pseudopregnancies, it was time to take the next step by examining Everett’s sperm. Three semen collection procedures from 2008 to 2011 yielded samples completely devoid of sperm! During the last collection, the veterinary staff collected tissue biopsies from each of Everett’s testicles and sent them to our Wildlife Disease Laboratories pathology group. After careful assessment of the tissue, the disappointing report and photos clearly indicated that Everett was not manufacturing sperm. The reason for his inability to produce gametes was unknown, but his infertility was now an indisputable fact.

Curators at both zoos arranged an exchange between San Diego and Santa Barbara, and Beau moved south to our Zoo. As Todd described, Beau and Anna took a little time to get to know each other but then began to breed. We were excited to assay Anna’s samples to see if the new pair would be successful. Following breeding in January of this year, Anna’s progesterone rose significantly, indicating that she had ovulated. Our hopes for a pregnancy, though, were again dashed when two months later her progesterone dropped back down to baseline.

But there was a very encouraging difference in this pseudopregnancy. This time Anna’s estrogen levels were twice as high as in previous pseudopregnancies and remained high for nearly one month in contrast to the rapid decline seen before. We are hopeful that very soon Anna will experience a normal pregnancy and have the opportunity to raise a litter of cubs. We’ll be watching from the lab.

Barbara Durrant is the Henshaw Director of Reproductive Physiology at the San Diego Zoo Institute for Conservation Research. Read her previous post, Meeting Hua Mei’s Son.

3

Elephant Serenade

Akaayla leads the group in a traditional African drum circle.

I love drumming. So, when I heard about the LivingSocial Drum Circle experience at the San Diego Zoo Safari Park, where I work, I had to get a ticket and see what it was all about. Not only would I get to play the drums, but I’d get to play them with members of our elephant herd as an audience! I wondered if the drumming would be bothersome to the herd or if it might actually bring out some sort of instinctual reaction to a sound that their species and family of origin were accustomed to. Only one way to find out! So I got my ticket to attend the May 19 drum circle event.

When I arrived for the event, I walked the path to the Safari Park’s Tembo Stadium and saw boxes and baskets full of instruments waiting to be played: drums, hammers (wooden sticks with round ends to beat the drums), shakers, and a variety of hand-made African instruments. I also saw and heard our own Dr. Zoolittle as he playfully chided people walking through or taking their seats. It was a great way to bring out the smile in everyone and prepare us for the experience we were about to have. Some of Dr. Zoolittle’s inquiries revealed that we were a very diverse audience, with guests visiting from places as far as Russia and Guyana to Los Angeles and Fresno and even Escondido, home of the Safari Park! But we were all there with a common interest: playing drums and appreciating the beauty of animals.

A variety of drums and shakers were available for use.

Dr. Zoolittle guided us as we collectively created the sound of African rain approaching, becoming heavier and then subsiding—all using just our hands. I closed my eyes and listened, and it was magic. I actually imagined myself being in South Africa with elephants and other animals listening in the distance. It was then that I knew this drum circle would prove to be even more than I expected.

Our leader for the more complicated portion of the drum circle was Akaayla, a professor at a San Diego university who teaches African drumming. What a privilege to be able to spend this time with her! She told stories of life in Africa, where women socialize by gathering together and clapping hands rhythmically, and how music and chanting were, in the truest sense of the word, instrumental in changing the political climate in South Africa during years of apartheid. She taught us, and we sang, a Soweto fight song that celebrated victory!

By the end of our time together making music and learning about Africa, the elephants that were previously at the farthest end of their yards had moved as close as they could get to where the drumming and singing was. And so, my initial question about how this event would be received by the elephants was answered.

I might never be able to visit Africa, though I hope to one day. This drum circle brought me close to it, and I have no doubt I’ll attend another. And I’m even more excited now about the arrival of the Summer Safari at the Safari Park that begins on June 30. Jambo!

Valerie Stoddard is a senior administrative assistant at the San Diego Zoo Safari Park. Read her previous post, Gorilla Exam Takes a Village.

17

Yun Zi: Busy Panda Boy

Yun Zi ponders his next move.

Yun Zi was definitely a busy boy on Saturday, May 19! The keepers gave him plenty of enrichment to keep him occupied. He received a tub full of ice, two hard-plastic Boomer balls filled with carrots, yams, apples, and herbivore biscuits, and a burlap sack filled with hay.

As soon as the bedroom door opened, our young panda ran to the tub and began to go through the ice, rubbing the cubes all over his head. He then tipped the tub over, spreading the ice everywhere, and tumbled down the hill, bringing the tub with him. Our guests were enthralled and were laughing and snapping as many photos as they could! Yun Zi even chased his Boomer ball around the exhibit, almost like a kid chasing a soccer ball.

After a lot of playing and running around for almost two hours, he FINALLY took a nap. What a day in the San Diego Zoo’s Panda Trek!

Alyssa Medeiros is a panda narrator at the San Diego Zoo. Read her previous post, Gao Gao and the Tub.

13

The Bears Thank You

Enrichment toys are vital for a recovering sun bear's health. Photo courtesy of BSBCC

Several months ago, we put out a call via our Animal Care Wish List asking for donations to provide enrichment items for the sun bears housed with our new collaborative partner, the Bornean Sun Bear Conservation Centre (BSBCC). You responded generously, and I am pleased to say we were able to send six new toys to the bears at the BSBCC. Thank you so much for your generosity!

The sun bear is a rare bear whose habitat is dwindling rapidly under pressure from deforestation. Primary causes of forest loss include illegal timber extraction and the development of palm oil plantations. Very few studies of wild sun bears have been conducted, and a population census of this species, or the Bornean subspecies, has never been conducted. However, their numbers must surely be on the decline as their habitat steadily shrinks.

One of my objectives is to find more opportunities to conduct research with sun bears, to learn more about them and facilitate conservation of this species. We have had the opportunity to observe the growth and development of four sun bear cubs born to our resident female, Marcella, but a larger sample size of animals was needed to conduct any statistically meaningful research into various aspects of their biology. Enter the BSBCC.

Siew Te Wong founded the BSBCC in Sabah, Borneo, to serve as a rescue and rehabilitation facility for orphaned and injured sun bears. “Wong,” as he is called, had conducted field work on these animals but recognized the need to provide care for bears impacted by forest loss and the illegal pet trade. In only 4 years of operation, the BSBCC has accumulated more than 20 sun bears. Some are destined for Wong’s developing reintroduction program, which will see them repatriated to the wild in time. Others are not good candidates for release and will likely live out their years at the BSBCC.

Thankfully, the BSBCC goes the extra mile to ensure a good home for its sun bears. It has several large outdoor pens that are essentially areas of enclosed natural habitat: giant trees, heavy canopy, soft forest soil, and a multitude of plants and bugs for the bears to enjoy. The enclosures are so natural that wild monkeys and birds often cruise in and perch in the canopy of their trees. The bears are carefully managed so that agreeable animals can be housed together as playmates when possible. Even so, there are so many of these animals that on any given day a few of the bears will be rotated inside so others can enjoy the outside spaces.

The BSBCC likes to provide enrichment for their indoor animals to ensure that their environment remains as stimulating as possible. And that’s where you come in. Your donations helped to aid in maintaining a quality of life for these bears that ensures their physical and emotional well-being. The photos here demonstrate that the bears are enjoying the toys immensely!

We are excited about developing our partnership with the BSBCC into a research opportunity. This will aid in the conservation of the smallest bear on Earth and could lend insight into the bear family tree. We know from our past work, for example, that sun bear mothers and panda mothers are very similar in their attentive maternal-care styles, and both pandas and sun bears differ from the less active hibernating bears like brown and black bears. What other similarities and differences between the bear species will we find?

Your gifts of enrichment were the first step in what I hope will be a long and informative road that leads to new discoveries about sun bears. Thank you again.

Suzanne Hall is a senior research technician for the San Diego Zoo Institute for Conservation Research. Read her previous post, Monday: Black, White, and the Blues.

5

Jaguar Cubs at Last!

Welcome, little jaguars!

About one year ago I wrote a blog post titled Jaguars: The Next Step. We had just recently introduced the San Diego Zoo’s jaguars with the hope of producing some much-needed cubs. As it turns out, the next step was the first step in a rollercoaster year that included hundreds of hours of behavioral observation, collection of more fecal samples than I care to remember, lots of amazing moments between the cats, and the heartbreak of an unsuccessful litter born in October.

Hold still! Each cub received a quick exam, including a weigh-in.

Over the course of the year we compiled a huge amount of jaguar data, some of which will be shared with other zoos all over the world to improve zoo-based breeding of this endangered cat. We saw our young, small Nindiri grow into an adult female, trading in some of her playful ways for more mature endeavors. We saw Guapo grow into a more confident animal as he figured out just how to get along with our always-spicy Nindiri. It was a year of many firsts for our jaguar friends and their keepers. We also achieved another milestone, something that we haven’t seen in San Diego for more than 20 years.

I have the privilege of announcing that a year’s worth of hard work, patience, and a major cooperative effort by people and jaguar has paid off. On April 26, Nindiri gave birth to two healthy, thriving little cubs, the first surviving jaguar cubs born at the San Diego Zoo since 1989. She quickly proved that she really has what it takes to be a great mother. Nindiri has been extremely attentive, opting to stay in her den box with her cubs nearly 24 hours a day. The few minutes away that she does take involve grabbing a few mouthfuls of food, a quick drink of water, and then back to her duties as mother.

There are some well-deserved privileges to being a jaguar keeper!

Our first official exam took place on their fourth day of life and involved getting a weight and a very quick all-over check by one of our vets. Much to my surprise, their eyes were already open. I hadn’t expected this to happen until they were at least a week old. Both cubs passed their quick exam with flying colors. The exam also gave us an early glimpse into personality. The first cub examined displayed some Nindiri-like attitude, hissing at me as I gently picked it up—a girl! The second cub, a boy, was quiet throughout the exam and seemed much less bothered by our imposition. Cleary cub #2 got his personality from Dad.

In the coming weeks will come the eating of solid foods, learning about our visitors, swimming lessons, figuring out how to get up into trees and—more importantly—just how to get down, and many, many other lessons that a jaguar cub must learn. Nindiri’s duties will continue to change throughout their many stages of life, and she has much to teach. It turns out that with these new cubs we are embarking on a new Next Step. A step toward a new, more maternal Nindiri. A step toward the next generation of jaguars. A step toward the conservation of this amazing species and ultimately a brighter future for the breeding of jaguars in our much-needed breeding programs. This next year will be another year of firsts, and I hope that you will share it with us.

Our new little residents will be off exhibit for a while, but look for them in Elephant Odyssey in the not-too-distant future.

Jacob Shanks is a senior keeper at the San Diego Zoo. Read his previous post, Zoo Conference: AZA.

1

Raising Maui Parrotbills

A newly hatched Maui parrotbill

The Hawaii Endangered Bird Conservation Program is pleased to announce our current success in raising the critically endangered Maui parrotbill (Hawaiian name: kiwikiu). This year, two chicks have hatched at the Maui Bird Conservation Center (MBCC), and one chick hatched at the Keauhou Bird Conservation Center (KBCC) on the Big Island. Our previous chick was raised in 2009, so adding three birds to the managed-care population over the course of one month is fantastic!

A newly hatched Maui parrotbill weighs only 1.5 grams (about the weight of a large paperclip!) and needs to be fed every hour between 6 a.m. and 8 p.m. for the first 13 days, with additional midnight feeds for the first 3 nights, which keep us very busy. Being intelligent and slow to become independent, Maui parrotbill chicks are susceptible to imprinting, so when their eyes begin to open, chicks are fed with a sock puppet created to look like the adult bird. When MBCC’s two chicks were old enough, we transferred them to KBCC so that they could all be together, helping them to develop the correct species identity.

A Maui parrotbill youngster

In the wild, Maui parrotbills form monogamous pairs that produce a clutch comprising a single egg. If raised successfully, the fledgling can remain with its parents for up to 17 months, so the species naturally has a low reproductive output. Here, we increase egg production by removing eggs from parental nests for artificial incubation, which can trigger the females to lay more eggs.

The Maui parrotbill is a member of the unique Hawaiian honeycreeper family. Currently, the Maui parrotbill’s range is extremely restricted to high elevation ohi`alehua forests on the eastern slopes of the Haleakala volcano on Maui. The wild population is estimated to be only around 500 birds. Although the population is currently considered to be stable, its distribution is limited primarily to one location, making it susceptible to extinction.

Growing chicks with a puppet "parent" watching over them

The Maui parrotbill is an insectivore that uses its strong, parrot-like beak to remove insect larvae from tree bark and fruit. Providing them with an extensive range of insects for their diet is a challenge, which we try to overcome by providing alternative nutritious foods and plenty of native branches for them to forage. In the last few weeks, we have started experimentally adding silkworms to the flock’s diet. We are hoping the bright yellow pigments contained in the green leaves eaten by the silkworms will ultimately be deposited in the birds’ plumage and enhance the yellow color of the males, making them more attractive to the females. With continuing effort and good fortune, we hope for another successful breeding season next year.

Amy Kilshaw is a research associate at the Maui Bird Conservation Center, part of the San Diego Zoo Hawaii Endangered Bird Conservation Program. Read her previous post, Nene Propagation: End of an Era.

20

Hello, Handsome Yun Zi!

Welcome back to the spotlight, Yun Zi!

It has been a couple of months, but I’m happy to report that giant panda Yun Zi is now on exhibit for guests to view. Sunday, May 13, was his first day back, and he was putting on quite a show for our lucky guests in the morning. A perfect Mother’s Day treat for our visiting mothers!

Since father Gao Gao was in that exhibit previously, Yun Zi spent the morning scent marking the entire exhibit. He also was running around and rolling around to show off and have fun. When the front viewing area was redone a couple of years ago, our keepers tried to keep panda youngsters in mind when they requested more climbing structures. Yun Zi is the perfect example of why that is so important for a young, growing panda. He was climbing, scent marking the tree and going all the way to the top to smell the air. It was really an amazing morning for our youngster!

Those who had not seen him in a while were shocked at how big Mr. Yun Zi has gotten. Currently, his weight is about 180 pounds, and he is looking like he is going to be rather tall as well. Since he is 2½ years old, he could potentially continue growing for a couple more years. But just like his parents, his weight can fluctuate with weather changes and different life changes (hormones). Many of our guests told me about being at the San Diego Zoo two years ago and seeing a little baby; they wondered what had happened to him. Just about all of them were astounded when I’d point to Yun Zi and say, “Here he is!”

If you get the chance, please come and visit him, and take a peak on the Panda Cam. As for mother Bai Yun, she has been moved into the north exhibit, which is currently closed to our guests, so that when we begin doing our thermal imaging on a regular basis she is easily accessible. Our first thermal imaging procedure has already taken place; Bai Yun cooperated beautifully, and we have nothing to report. Please remember that it can take a while for our researchers and vet staff to see anything that would indicate a pregnancy. Paws crossed!

Anastasia Horning is a panda narrator at the San Diego Zoo. Read her previous post, Panda Narrator at Safari Park.

0

The Last Ones?

Panamanian golden frog

If you sat next to me on the plane traveling home from Panama this past February, you probably thought that my tote bag was full of souvenirs from a grand, tropical vacation. Instead, I was carrying the carefully preserved and packaged bodies of endangered frogs from captive survival-assurance populations. This was a trip that required months of careful planning and lots of red tape in obtaining and using the complicated permits needed to transport wildlife samples. Far from being morbid, icky, or gross, these specimens were extremely valuable for scientific efforts to save amphibian species from extinction. So why would anyone willingly travel with dead frogs?

Does Allan's yellow tote bag hold hope for amphibian species?

To explain, I should tell you that I’m a veterinarian who specializes in pathology. Therefore, my day-to-day responsibilities are focused on using laboratory techniques, including necropsies (animal autopsies), to accurately diagnose disease in animals at the San Diego Zoo and the San Diego Zoo Safari Park as well as our field conservation programs. Through these activities, our Wildlife Disease Laboratories have a mission to remove disease as a roadblock to wildlife conservation. By bringing these deceased frogs to our laboratory and sleuthing out their parasites and disease problems, we hope to make useful recommendations that can help improve things like animal diets or aid veterinarians in selecting the very best treatments. Ultimately, this helps to ensure that the captive populations can be sustained and thrive until they can someday return to the wild.

Promoting the success of amphibian survival assurance populations is no trivial matter: more than one third of the world’s approximately 6,000 amphibian species are in decline because of introduced disease, loss of habitat, environmental change, and human exploitation. Although sometimes I get wrapped up in dry scientific and technical details, this group of frogs from Panama now in my bag really reminded me of why I do what I do.

Allan holds some of the carefully preserved frog specimens for study.

Among these specimens were species like the Panamanian golden frog, which soon may survive only in captive survival assurance populations, and the fringe-limbed tree frog, for which only a single individual is still known to exist. It is difficult to describe the feeling of holding what may be the last individuals of an entire species in your hand, but I can tell you that it hit hard for me, and I know that it is worse for friends and colleagues on the front lines of the amphibian decline who don’t have the luxury of retreating into the laboratory.

I am privileged to work for a unique organization that recognizes the importance of what might seem like an unusual scholarly activity. Collaborating with colleagues nationally and internationally really makes amphibian conservation happen! I also have the support of an amazing team in the Wildlife Disease Laboratories who will move mountains if they think it will help animals in need.

If you’d like to know more about the amphibian extinction crisis and what you can do to help, please visit the Amphibian Ark® online at www.amphibianark.org. Some of the most important actions for saving amphibian species, like protecting the environment and raising awareness of the plight of animals, can happen from within our homes.

Allan Pessier is a senior scientist for the Wildlife Disease Laboratories, San Diego Zoo Institute for Conservation Research.

2

Patience: A Porcine Virtue?

Our photographer caught one piglet at rest...for the moment!

At 2 p.m. on March 27, Goldie, a red river hog living in Nairobi Village at the San Diego Zoo Safari Park, delivered four healthy, rambunctious piglets. For four months, we had watched expectantly as Goldie’s belly had grown bigger and bigger (round even by pig standards!). The piglets, two boys and two girls, were instantly active. Red river hogs are naturally precocious, and these four are no exception. The boys, Baloo and Bagheera, take their names from The Jungle Book like their dad, Mowgli. The girls, Zamu and Zola, have Swahili names like their older sister, Lozi.

Anytime the four piglets are awake, they are in motion. Whether sparring and chasing, rooting and digging up their exhibit, or climbing over Mom and Dad, the little siblings are constantly busy. This much activity raises one question: how do the parents and big sister tolerate the four tiny tornadoes? The answer is with great patience: porcine patience.

This being her third litter, Goldie knows how to handle seemingly tireless piglets who want to eat all the time. Any time Goldie stops walking, the piglets latch on to suckle. Eventually, Goldie flops to her side to allow them to nurse. Older sister Lozi is turning out to be a terrific role model. She never snaps at her striped siblings, even when they steal food out from under her snout.

Father Mowgli is a paragon of patience; his calm, collected gait is hardly altered as the piglets dart between his legs. He tolerates the piglets’ constant pushing, shoving, and climbing. And it would seem the piglets are even learning to take it easy from him. Usually the last one to leave a comfortable bed in the morning, he is now joined by his black-and-orange offspring. Goldie and Lozi jolt awake at the sound of breakfast; Mowgli and kids lay sleepily in their hay a few minutes longer. It’s not uncommon to find the piglets nestled snugly against Dad…when they choose to slow down, that is.

Interested in all the activity at the red river hog exhibit? Visit the Safari Park and watch the piglets as they root, dig, forage, and even learn to show a little patience.

Matt Gelvin is a senior keeper at the San Diego Zoo Safari Park.

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Panda Narrator at Safari Park

Guests on a Rolling Safari get a unique view of the Park's lions!

One of the great benefits of working for San Diego Zoo Global is that it gives employees the opportunity to explore other positions in the organization. For some of us, we can go on loan to other departments to help out during busy times. Although I am a panda narrator at the Zoo, for about three years now during Spring Break weeks I have had the great opportunity to work at the Safari Park and help give Africa Tram Safari and Rolling Safari tours. The best part of this process is learning about the different conservation programs that we are doing on grounds at the Park and letting our guests know that by coming to the Park they have helped us in all of our endeavors.

On the Africa Tram Safari, some of the first animals our guests see are the South African cheetahs. The Park has 1,800 acres, so we have space for a cheetah breeding facility where we have welcomed over 130 cheetah births. Many of our cheetahs born here become ambassadors for their species and go to other zoos to help bring awareness to their plight in the wild.

Southern white rhinos can roam far and wide at the Safari Park.

One of the most famous animals at the Park is the southern white rhino. In the early 1900s, the rhinos were hunted for their horns to the point where there were less than 500. For years, the practice in zoos was to put a male and a female rhino together and wait for them to breed. We now know that southern white rhinos are social animals, and the females like to be in a herd to help protect their babies. They won’t even come into heat unless they have that social group! Right now we have a male at the Park chasing the girls around, trying to court. The funniest thing about these animals is that if a male wants to court a female, he has to get the approval of the rest of the herd!

On the Rolling Safari Tours, our guests get to ride on a Segway X2 while getting to see the Asian animals that are not out for general viewing, such as the greater one-horned rhino, Przewalski’s horse (an extremely rare wild horse), Indian gaur (one of the few wild cattle left), and the Arabian oryx (an animal that was extinct in the wild but was brought back due, in part, to the Safari Park’s breeding efforts).

Taking a Safari Park tour is a great way to really see how involved San Diego Zoo Global is with conservation. Every tour lets you get extra information about what we do, our mission, and how you have helped and can continue to help as you leave the Park. I think the most exciting thing for some is seeing the new babies born. Since the Park opened in 1972, over 20,000 animals have been born here, and we help the scientific community by sharing our knowledge of animal behavior, successful techniques to secure breeding, and successful animal management.

If you have the time, please come check out the Zoo’s sister facility, the Safari Park. Watch these animals interact with each other in the large, open exhibits, and see animal behavior like you’ve never seen before.

Anastasia Horning is a panda narrator at the San Diego Zoo. Read her previous post, Our Growing Takin Calves.